A: No pre-treatment is necessary: Seed should germinate upon sowing in a warm location.
B: Hot water treatment: This hot water treatment helps break open the hard seed coat. This may happen naturally with freeze/thaw cycles, but better germination can be expected if hot water treatment is done before fall planting outside, or artificial cold-moist stratification in a fridge (Germination Code C).
1. Bring water to a boil, remove from heat, and pour over seeds.
2. Soak the seeds at room temperature for 24 hours.
3. Filter through a coffee filter and fine mesh strainer.
4-5. Proceed with germination code C instructions (below).
C (# of stratifying days): Cold, moist stratification needed: To naturally stratify seed, plant outdoors late fall on a weed-free site and allow seed to overwinter. To artificially stratify seed, place seed and medium in a labeled, sealed plastic bag and store in a refrigerator (33-40°F). Stratification medium could be a damp paper towel, coffee filter, sand, vermiculite, or other horticultural-use medium.
D: Seeds are very small or need light to naturally break dormancy and germinate: Seed requiring this treatment should be surface-sown. No soil cover, or just a dusting of soil, should be applied. If grown in outdoor beds, sow stratified (if required) seed on a level surface. Cover with a single layer of burlap or cotton sheet to help retain moisture at the soil surface. Remove cover after germination. Do not let soil dry out until seedlings are established. Shading with a window screen set 12” above the soil during the first season will also help prevent drying. If sowing seeds in containers, water from the bottom as necessary.
E: In order to germinate, seeds need a warm, moist period (summer) followed by a cold, moist period (winter): Sow outdoors in spring and allow one full year for germination. To artificially start this stratification process, mix seeds with horticultural-use medium, place mixture in a labeled, sealed, plastic bag and store in a warm place (about 80°F) for 60–90 days. Then place in your refrigerator (33–40°F) for 60–90 days before sowing.
F: Seeds need a cold, moist period (winter) followed by a warm, moist period (summer) followed by a 2nd cold, moist period: Sow outdoors and allow 2 years or longer to germinate. To artificially start this stratification process, follow the instructions for Germination Code C for 60-90 days, then store in a warm (about 80°F) place for 60-90 days, followed by a 2nd cold period in the refrigerator.
G: Seeds germinate most successfully in cool soil: Sow seeds in late fall (after hard frost) or early spring.
H: Seeds need scarification: These species require weakening of the seed coat in order to come out of dormancy. Scarify by rubbing seed between two sheets of medium-grit sandpaper. The goal is to abrade seed coats – stop if seeds are being crushed. Scarification should be done before stratification (Germination Code C) if needed. Fall or winter sown seed should not be scarified to prevent the chance of premature germination and winter kill.
I: Legume (member of the pea family): Most legume species harbor beneficial bacteria called rhizobia on their roots. Genus-specific strains of this bacterium called inoculum can aid in the fixation of atmospheric nitrogen and improve long-term health of native plant communities. Inoculum is naturally occurring in most soils and additional amendments are not needed.
K: Hemiparasitic species which requires a host plant: Good hosts for many hemiparasitic species include low-growing grasses and sedges like Blue Grama, Pennsylvania Sedge, Little Bluestem, and June Grass. With a knife, make a 2” deep cut at the base of the host plant. Sow seed in the cut, making sure seed is not more than 1/8” deep. If host is transplanted at sowing time, the cut is not needed because damaged roots will be available for attachment by the hemiparasite. You may also try sowing hemiparasitic and host species seeds together at the same time. To add hemiparasitic species to existing sites, scatter seed on soil surface (rake in if seed is large) in late fall.
L: Plant fresh seed or keep moist: Refrigerate until planting or starting other treatment.
M: Best planted outdoors in the fall: Artificial stratification may not work well.
O: Impermeable seed coat: Nick seed coat with knife, and soak in water overnight before planting.